Biological Sciences Tutorial: CryoEM with Phase Plates

Applicability of thin film phase plates in biological electron microscopy

Presented is an evaluation of phase contrast techniques in transmission electron microscopy. The traditional defocus phase contrast is compared to two recently developed phase plate techniques. One is the Zernike phase contrast transmission electron microscope, the other is the Hilbert differential contrast thransmission electron microscope. The imaging characteristics of each technique are dis...

Phase contrast electron microscopy: development of thin-film phase plates and biological applications

Phase contrast transmission electron microscopy (TEM) based on thin-film phase plates has been developed and applied to biological systems. Currently, development is focused on two techniques that employ two different types of phase plates. The first technique uses a Zernike phase plate, which is made of a uniform amorphous carbon film that completely covers the aperture of an objective lens an...

Medical and Biological sciences

group of 116 women for comparison has daughters with physiological flowing of pubescence period. The age of first menstruation and a character of the beginning of the women’s menstrual function in the comparative groups aren’t greatly different. Real difference in the clinic demonstration of diseases of ears, noses, threats organs 57(49,1%) the daughters have their broken menstrual function and...

Classification: Biological Sciences, Psychology

Biological Sciences: Biophysics

In this study, we explore nucleation and the transition state ensemble of the ribosomal protein S6 using a Monte Carlo Go model in conjunction with restraints from experiment. The results are analyzed in the context of extensive experimental and evolutionary data. The roles of individual residues in the folding nucleus are identified and the order of events in the S6 folding mechanism is explor...